|The following is a list of main and recurring characters in the Oobi series.
- Oobi is a clever hand puppet who loves to learn and explore. He is puppeteered by Tim Lagasse.
- Uma is Oobi's excitable younger sister. She is puppeteered by Stephanie D'Abruzzo.
- Grampu is Oobi and Uma's grandfather, who loves to cook. He is puppeteered by Tyler Bunch.
- Kako is Oobi's best friend. He is puppeteered by Noel MacNeal.
- Inka is Oobi's piano teacher. Grampu shows affection for her in several episodes.
- Angus is one of Oobi's friends, who is often nervous to step out of his comfort zone.
- Mrs. Johnson is an elderly woman and one of Oobi's neighbors.
- Maestru is a conductor who wears a bow tie.
- Frieda is a foot whom Oobi plays with at the park on several occasions.